In vitromaturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
نویسندگان
چکیده
منابع مشابه
In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's ...
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هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Cysteamine, glutathione and ionomycin treatments improve in vitro fertilization of prepubertal goat oocytes.
The aim of this study was to improve in vitro embryo development of prepubertal goat oocytes by studying the effect of adding cysteamine to in vitro maturation medium, glutathione (GSH) to in vitro fertilization medium and ionomycin to the sperm capacitation medium. In experiment 1, we analysed the effect of 1 mM GSH added to fertilization medium of oocytes matured with 400 microM cysteamine. T...
متن کاملQuality of oocytes in prepubertal and pubertal swine
Rita GRABOWSKA*, Barbara BŁASZCZYK, Tomasz STANKIEWICZ, Tomasz BANAŚ, Sarah HALE, Jan UDAŁA Nuffield Division of Clinical Laboratory Science, University of Oxford, John Radcliffe Hospital, Oxford, UK Department of Gynecology and Oncology, Jagiellonian University, Krakow, Poland Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian Technological University...
متن کاملCharacterization of Black Bengal goat
Characterization of Black Bengal bucks and does was performed in this study. A total of 50 goats of different aged were studied. The experiment was performed in Black Bengal bucks and does at 1 day and at 3, 6, 9 and 12 months of age for measuring different parameters such as body length, heart girth, wither height, hip height, fore and hind leg length, head length and width, head length and wi...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2011
ISSN: 1229-845X
DOI: 10.4142/jvs.2011.12.1.75